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I wanted to add to what cgduncan mentioned above about hcg levels post-methotrexate - my doctor was very clear that she usually sees the HCG levels rise in the first follow-up blood test (3 days after the shot). She speculated that when the methotrexate starts to work, the pregnancy gives a last hurrah burst of HCG.
Then for the test 1 week after the shot, she wants to see the number go down at least 15% from the 3 day draw.
I'm sure everyone's different, but I wanted to throw this in for those whose hcg numbers don't go down in that first test.
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Our Team:
me: 40 with unexplained infertility
dp (dear partner): 46
ttc 2 years
donors (yes, 2) - both fabulous
4/06 - very 1st IUI - BFP ; m/c and d&c at 8 wks
10/06 - 3rd IUI - BFP - but chemical and lost at 5+ weeks
6 more IUIs - BFN all
8/07 Surgery to remove ovarian cyst
10/07 1st IVF - BFP; ectopic - d&c and methotrexate 
3/08 IVF with donated eggs
3/31 -  ! beta 95; 4/2 - beta 267  ; 4/7 - beta 2073!!!
4/8 - early u/s - a perfect little dot in its perfect little spot!
4/15 - bleeding scare, but saw the bean measuring perfectly and beating its wee heart!
5/2 - u/s - all's well and hb at 175
7/9 - anatomy scan - all's fabulous!
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