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Martie-when are you going to learn not to ask questions that you don't want the answer to? Transformation...I am putting a plasmid containing a toxin gene into bacterial cells. This is done by the heat shock method. See, previously these cells have been made 'competent' by CaCl2. This kind of pokes small holes in the cell's membrane so large pieces of circular DNA can enter the cell. Heating them allows this to occur. Then I gorw the cells up (as I am doing right now), then plate them on LB/Carb (antibiotic for selection) so I can grow single colonies... Sorry you are not getting any visitors this weekend ![]()
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![]() Me: 35 MTHFR hetero, no other issues DH: 33 Perfect Etienne , Jake , Sam (cats) Maggie (iguana)TTC #1: 2 yrs 2 m/c (Aug '06 and Jan '07), 1 ectopic pg (lost left tube) 5 IUI's 5/27: beta #1: 716!!!! (17dpo) Natural cycle!5/29: beta #2: 1885!!!! 6/1: early u/s-1 sac/yolk 6/9: u/s#2-saw and heard hb! 114 bpm. Measuring 6w2d 6/17: u/s #3- hb 154 bpm. Measuring 7w3d 7/1: u/s #4- hb 161 bpm. Measuring 9w4d 7/9: first OB appt.-u/s #5- hb 152 bpm. Measuring 10w4d 7/17-NT scan-everything looks great! Measuring 11w6d 8/14-quad screen (quick gender check): IT'S A BOY!!! 9/4- Level II u/s. Baby is doing great! Measuring 5 days ahead. ![]() ![]() check out my website! www.glassphusions.wordpress.com |
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Martie, is it good or bad to have the house without visitors?
Can you believe all the love and good energy on this thread!? I love it. |
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Martie- That sucks about your visitors!! Hope your MIL and neice are feeling better soon!!
Hey Jen- we say y'all up here too. I use it all the time. Hee hee. My mom makes fun of the way I talk, I guess I've gotten rather lazy with pronunciations since we moved. She is always asking me to repeat myself cause she can't understand what I'm saying. LOL Like the other night she claims I said MATformin instead of METformin. I think she's crazy. Oh and I say muffins weird too! LOL
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~Shawna~ Me: 26(Borderline PCOS) DH: 25 (slight MF) TTC: Oct/04 IVF: -10 fertilized, 6 Ice Babies -July 14th Transfer of two beautiful little guys. : Boo yah!!! July 21st for sure...it's getting darker!God please stay with us and this miracle. Beta: July 27-341 July 29-680 First u/s aug 18-IT'S TWINS! Baby A (Monkey) and Baby B (Boo) both with Heart rates around 160 bpm. 10w2d u/s- Sept 8: Looking good 10w6d-spotting scare 11w6d- NT scan shows 2 beautiful growing babies Gender and Anatomy: Nov 20th Mommy's intuition was right: TWO HEALTHY BOYS!! Angel babies: Bean lost 12/11/06 at 8 weeks EDD July 23/07 Peanut lost 6/8/07 at 4 1/2 weeks EDD Feb 11/08 Little Ray lost 03/13/08 Ectopic at 6w5d EDD Oct31/08
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Ugg I swear my hair looks like something out of the late 70s early 80s right now. All light and fluffy....I wish I could mke my hair look nice EVERY day...instead of once in a blue moon! LOL
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~Shawna~ Me: 26(Borderline PCOS) DH: 25 (slight MF) TTC: Oct/04 IVF: -10 fertilized, 6 Ice Babies -July 14th Transfer of two beautiful little guys. : Boo yah!!! July 21st for sure...it's getting darker!God please stay with us and this miracle. Beta: July 27-341 July 29-680 First u/s aug 18-IT'S TWINS! Baby A (Monkey) and Baby B (Boo) both with Heart rates around 160 bpm. 10w2d u/s- Sept 8: Looking good 10w6d-spotting scare 11w6d- NT scan shows 2 beautiful growing babies Gender and Anatomy: Nov 20th Mommy's intuition was right: TWO HEALTHY BOYS!! Angel babies: Bean lost 12/11/06 at 8 weeks EDD July 23/07 Peanut lost 6/8/07 at 4 1/2 weeks EDD Feb 11/08 Little Ray lost 03/13/08 Ectopic at 6w5d EDD Oct31/08
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I feel bad for the reasons they are not coming. But I not totally upset if you know what I mean.
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Sept. 2004 ectopic pregnancy (lost left tube) 2006 4 IUI no success May 2007 1st IVF *pregnancy but lost on 6/24/07 at 8 weeks 3 days Sept 2007 FET 9/07 AF is here here we go again!!9/10 screen scan ultrasound 9/11 start 75 iu of Gonal F 9/17 BW and ultrasound (everything going along fine increase gonal f to 150iu) 9/20 bw and ultrasound (lining at 12mm, but eggs only went from 12 to 14mm, e2 not high enough) 9/22 bw and ultrasound(everything grew nicely 1 more night of meds!!!!) 9/23 trigger 9/27 ET (3 frozen embryos) 10/8 beta 21 ![]() ![]() ![]() 10/10 beta 51 ![]() ![]() 10/22 ultrasound (1 little Jelly Bean) 11/05 2nd ultrasound (heart beat 170 bpm) 11/12 3rd u/s due to spotting (all is well h/b 185 bpm) EDD June 18, 2008 ![]() |
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Martie- I DO know what you mean! hee hee. It sucks but it doesn't. i hear you. I;m the same with my family.
Okay ladies, does this sound stupid? But I am thinking of getting two words tattooed on my wrists. (one each wrist) On one side the word "Faith" the other the word "Love". Then I thought about adding three little stars for each little one we've lost. So a red, purple and now pink star. (probably a nautical star) but I don't know which word to put the stars under. Faith because they give remind to have it in myself, them and my DH, as well as my God. Or love because I loved them all so much even though they were only with us for such a short time. As well, I have to convince DH that wrist tattoos are cool for me! LOL I really want them. I am also hoping to get my nose pierced that's topic for some other time. What do you all think?
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~Shawna~ Me: 26(Borderline PCOS) DH: 25 (slight MF) TTC: Oct/04 IVF: -10 fertilized, 6 Ice Babies -July 14th Transfer of two beautiful little guys. : Boo yah!!! July 21st for sure...it's getting darker!God please stay with us and this miracle. Beta: July 27-341 July 29-680 First u/s aug 18-IT'S TWINS! Baby A (Monkey) and Baby B (Boo) both with Heart rates around 160 bpm. 10w2d u/s- Sept 8: Looking good 10w6d-spotting scare 11w6d- NT scan shows 2 beautiful growing babies Gender and Anatomy: Nov 20th Mommy's intuition was right: TWO HEALTHY BOYS!! Angel babies: Bean lost 12/11/06 at 8 weeks EDD July 23/07 Peanut lost 6/8/07 at 4 1/2 weeks EDD Feb 11/08 Little Ray lost 03/13/08 Ectopic at 6w5d EDD Oct31/08
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personally, I think you are crazy Shawna, but that is why I love you so much!
Just thinking about the pain of a needle going into my wrist...I can't even talk about it! But the idea is sweet. And definitely get Dh's permission (well, get him to agree) cause you don't want him pissed at you for this!
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![]() Me: 35 MTHFR hetero, no other issues DH: 33 Perfect Etienne , Jake , Sam (cats) Maggie (iguana)TTC #1: 2 yrs 2 m/c (Aug '06 and Jan '07), 1 ectopic pg (lost left tube) 5 IUI's 5/27: beta #1: 716!!!! (17dpo) Natural cycle!5/29: beta #2: 1885!!!! 6/1: early u/s-1 sac/yolk 6/9: u/s#2-saw and heard hb! 114 bpm. Measuring 6w2d 6/17: u/s #3- hb 154 bpm. Measuring 7w3d 7/1: u/s #4- hb 161 bpm. Measuring 9w4d 7/9: first OB appt.-u/s #5- hb 152 bpm. Measuring 10w4d 7/17-NT scan-everything looks great! Measuring 11w6d 8/14-quad screen (quick gender check): IT'S A BOY!!! 9/4- Level II u/s. Baby is doing great! Measuring 5 days ahead. ![]() ![]() check out my website! www.glassphusions.wordpress.com |
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I definately take that as a compliment Jen.
I like being crazy! Hee hee. I'm actually more freaked out about piercing my nose then getting those tattooes. Funny hey?! Of course I would never just go off and come home all inked up, but it is tempting! lol. I'm sure he just needs time to come to terms with it. lol
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~Shawna~ Me: 26(Borderline PCOS) DH: 25 (slight MF) TTC: Oct/04 IVF: -10 fertilized, 6 Ice Babies -July 14th Transfer of two beautiful little guys. : Boo yah!!! July 21st for sure...it's getting darker!God please stay with us and this miracle. Beta: July 27-341 July 29-680 First u/s aug 18-IT'S TWINS! Baby A (Monkey) and Baby B (Boo) both with Heart rates around 160 bpm. 10w2d u/s- Sept 8: Looking good 10w6d-spotting scare 11w6d- NT scan shows 2 beautiful growing babies Gender and Anatomy: Nov 20th Mommy's intuition was right: TWO HEALTHY BOYS!! Angel babies: Bean lost 12/11/06 at 8 weeks EDD July 23/07 Peanut lost 6/8/07 at 4 1/2 weeks EDD Feb 11/08 Little Ray lost 03/13/08 Ectopic at 6w5d EDD Oct31/08
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how about draw what you want on your wrists with marker and let him 'notice' it....then wait for reactions?
and you are crazy in the best way! I love you Shawna!!! ![]()
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![]() Me: 35 MTHFR hetero, no other issues DH: 33 Perfect Etienne , Jake , Sam (cats) Maggie (iguana)TTC #1: 2 yrs 2 m/c (Aug '06 and Jan '07), 1 ectopic pg (lost left tube) 5 IUI's 5/27: beta #1: 716!!!! (17dpo) Natural cycle!5/29: beta #2: 1885!!!! 6/1: early u/s-1 sac/yolk 6/9: u/s#2-saw and heard hb! 114 bpm. Measuring 6w2d 6/17: u/s #3- hb 154 bpm. Measuring 7w3d 7/1: u/s #4- hb 161 bpm. Measuring 9w4d 7/9: first OB appt.-u/s #5- hb 152 bpm. Measuring 10w4d 7/17-NT scan-everything looks great! Measuring 11w6d 8/14-quad screen (quick gender check): IT'S A BOY!!! 9/4- Level II u/s. Baby is doing great! Measuring 5 days ahead. ![]() ![]() check out my website! www.glassphusions.wordpress.com |
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Well you know that the love is mutual!
And that I enjoy being crazy! ![]() Ya know it's funny that you should mention drawing it on for DH to see, he has done that with all the tattooes I've wanted to get. LOL. So some of them have been shot down while others are still in the works. lol
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~Shawna~ Me: 26(Borderline PCOS) DH: 25 (slight MF) TTC: Oct/04 IVF: -10 fertilized, 6 Ice Babies -July 14th Transfer of two beautiful little guys. : Boo yah!!! July 21st for sure...it's getting darker!God please stay with us and this miracle. Beta: July 27-341 July 29-680 First u/s aug 18-IT'S TWINS! Baby A (Monkey) and Baby B (Boo) both with Heart rates around 160 bpm. 10w2d u/s- Sept 8: Looking good 10w6d-spotting scare 11w6d- NT scan shows 2 beautiful growing babies Gender and Anatomy: Nov 20th Mommy's intuition was right: TWO HEALTHY BOYS!! Angel babies: Bean lost 12/11/06 at 8 weeks EDD July 23/07 Peanut lost 6/8/07 at 4 1/2 weeks EDD Feb 11/08 Little Ray lost 03/13/08 Ectopic at 6w5d EDD Oct31/08
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Shawna, that gives a new meaning to wearing your heart on your sleeve :-)
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Me (35) Lap found Endo in 07/07; diagnosed Hashimoto's (Lymphocytic Thyroiditis) & MTHFR Hetero 05/08 ~ DH has slight MF ~ 2 kitties ~ MY BLOGIVF 3 - Take 2 Nov/Dec '08 @ CCRM.........................IVF#2 Jan '08 - BFN 8/22 TSH= 19 + P4= 2.4 at suppression check ........Antagonist: 7 eggs, 1 mat, ICSI, AH, 1 Fert; 3dt of 2 7-cells + 2 6-cells (used frosties) 9/05 TSH= 3 w/high thyroglob. antibodies .............IVF#1 Sept '07 - BFN 9/13 Dr. says wait 6 wks to see if TSH = 1 ...............Antagonist: 10 eggs, 6 mat, ICSI, AH, 5 fert; 3dt of 2 great 8-cells (froze 3 lesser) 10/23 CD1 and TSH@1.87 11/22 with AF, start Microdose Lupron cycle CD1 CD2 u/s + b/w CD3 lupron 20 / start dexamethasone CD4 lupron 20 CD5 menopur AM + follistim PM Going to try for CGH in December with a FET of gorgeous embies in February! |
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It would wouldn't it?!
I keep telling DH I want to be THAT cool tatted Auntie for all our neices and nephews. I think his sisters just think I'm a freak and unhealthy though since they oddly have this huge thing against tattooes in their family. My MIL was shocked when we came home with tatts from hawaii. lol.
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~Shawna~ Me: 26(Borderline PCOS) DH: 25 (slight MF) TTC: Oct/04 IVF: -10 fertilized, 6 Ice Babies -July 14th Transfer of two beautiful little guys. : Boo yah!!! July 21st for sure...it's getting darker!God please stay with us and this miracle. Beta: July 27-341 July 29-680 First u/s aug 18-IT'S TWINS! Baby A (Monkey) and Baby B (Boo) both with Heart rates around 160 bpm. 10w2d u/s- Sept 8: Looking good 10w6d-spotting scare 11w6d- NT scan shows 2 beautiful growing babies Gender and Anatomy: Nov 20th Mommy's intuition was right: TWO HEALTHY BOYS!! Angel babies: Bean lost 12/11/06 at 8 weeks EDD July 23/07 Peanut lost 6/8/07 at 4 1/2 weeks EDD Feb 11/08 Little Ray lost 03/13/08 Ectopic at 6w5d EDD Oct31/08
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Shawna..What about having your belly button peirced? Or do you have that already? Jen..You are so smart! When you start talking all scientific, I read the first word or two and then it's like Charlie Brown's teacher...Waa..waa waa..waa! Do you remember that? lol! Anyway, I'm impressed!
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![]() Me 41 April DP 41 Fur babies: Bella 3 yr old lab and Dino cat3/07 IUI cancelled due to poor response IVF #1 bfn IVF #2 converted to IUI-bfn IVF #3 bfn FET #1 bfn 5 Frozen Embies Aug. '08 IVF #4 DP is up to bat! 08/16 ER 22 eggs/17fertilized! 08/21 transfered 4 blasts ![]() 5dp5dt faint + 8dp5dt + 09/02 Beta #1 844! 09/04 Beta #2 1977 09/18 1st u/s It's twins!! Baby A hb 118 and Baby B hb 125 09/25 u/s #2 Baby A's hb 160 and B's 146 10/05 u/s #3 Baby A's hb 167 and B's 174 We're having a Boy and a Girl!! ![]() |
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Once upon a time, April, hee hee...I had 13 piercings. One of which was my belly button. I still have the scar too. boo. lol. But I think button piercing is out, cause then I'd just have to take it out after I get PG again when this time have a healthy one. lol.
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~Shawna~ Me: 26(Borderline PCOS) DH: 25 (slight MF) TTC: Oct/04 IVF: -10 fertilized, 6 Ice Babies -July 14th Transfer of two beautiful little guys. : Boo yah!!! July 21st for sure...it's getting darker!God please stay with us and this miracle. Beta: July 27-341 July 29-680 First u/s aug 18-IT'S TWINS! Baby A (Monkey) and Baby B (Boo) both with Heart rates around 160 bpm. 10w2d u/s- Sept 8: Looking good 10w6d-spotting scare 11w6d- NT scan shows 2 beautiful growing babies Gender and Anatomy: Nov 20th Mommy's intuition was right: TWO HEALTHY BOYS!! Angel babies: Bean lost 12/11/06 at 8 weeks EDD July 23/07 Peanut lost 6/8/07 at 4 1/2 weeks EDD Feb 11/08 Little Ray lost 03/13/08 Ectopic at 6w5d EDD Oct31/08
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